Kohler illumination

From emsuite wiki

Köhler Illumination

Kohler illumination is a process of aligning the light source at the focal plane you will be using with your microscope.



Adjust the alignment of the lamp according to the microscopes manufacturers specifications.

Step 1.

Find the specimen and bring it into the best focus you can achieve. At this time adjust  both oculars for you vision. Looking through the oculars close your weakest eye and while using your strongest eye focus that ocular with the adjustment collar at the neck of the ocular. With both eyes opened focus the other ocular.

Step 2.

Close the field diaphragm as much as you can, this aperture is the closest to the lamp. Adjust the condenser height so what you see is the smallest and sharpest edged aperture opening.

Step3. 

Open the field diaphragm until almost all the field is illuminated. Center the aperture  so the light is the same distance from all the edges. Now open the diaphragm only until the whole visible  field of view is illuminated.

Step 4.

Close the aperture diaphragm of the condenser so only b of the back of the objective lens is visible.